Nucleic Acid Controls
Sigma Aldrich Fine Chemicals Biosciences DNA Calf Thymus activated
DNA (Calf Thymus)Nonmethylated DNA (at a concentration of 500 mug/ml) for preparation of molecular weight markers. Methylated DNA and nonmethylated DNA (at a concentration of 500 mug/ml) for determining methylation sensitivity of restriction enzymes. High-quality DNAs from viral and eukaryotic sources used for preparation of assay reagents. Activated calf thymus DNA at 25 A260 mu/ml (treated briefly with DNase I)-for preparing substrate used in the study of DNA polymerases.
Sigma Aldrich Fine Chemicals Biosciences Calf Thymus Sonicated DNA
Genomic DNA sheared by sonication for use in hybridization experiments.Tested for absence of nuclease. Fragmented DNA with a size range from 200-6000 nucleotides. Purified by phenol extraction and freeze dried. Suitable for blocking non-specific binding sites in hybridization experiments to reduce background. DNA isolated from Calf thymus is sonicated to fragment the DNA to an average size of approximately 400 bp.